Single‑Dose Study
Lower mental-stress and tension–anxiety scores vs. placebo*
Measured within 60 minutes of a single dose
Read study →The Science Behind PressurePrep
Composure when you need it. Resilience when you build it.
Stress isn’t only a momentary problem. PressurePrep was designed around two goals: support calm, focused attention when pressure is high—and support a more resilient response to everyday stress with consistent use over time.
We started with clinically studied L‑theanine, already widely used in the U.S. for calm, focused attention. Then we searched globally for ingredients with something harder to find: meaningful human clinical evidence for both short‑term composure and longer‑term stress resilience.
That led us to L. gasseri CP2305, a Japanese postbiotic studied for acute and sustained stress response, and CEREBIOME®, a proprietary probiotic combination studied in humans during periods of ongoing everyday stress.
40+
Randomized human trials across three core ingredients
L‑Theanine
31 randomized trials
1,168 participants
L. gasseri CP2305
8+ randomized human trials
Acute + sustained‑use research
CEREBIOME®
9 human clinical studies
5+ randomized trials
Studies cited on this page evaluated the individual ingredients or specific strains—not the finished PressurePrep formula. Individual results may vary.
Two time horizons. Three clinically studied ingredients.
In acute human studies, within 60 minutes L‑theanine improved attention and focus and lowered salivary cortisol, while CP2305 increased alpha‑wave activity and parasympathetic activity and lowered mental‑stress and tension–anxiety scores.*
With consistent daily use, CEREBIOME® reduced cortisol levels and improved psychological‑distress measures. In medical students subjected to sustained high pressure over time, CP2305 reduced anxiety and sleep disturbance.*
Studies cited on this page evaluated the individual ingredients or specific strains—not the finished PressurePrep formula. Individual results may vary.
*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.
L‑Theanine
A familiar starting point.
31 Randomized Trials · 1,168 ParticipantsL‑theanine is widely used for calm, focused attention without sedation. A recent systematic review and meta‑analysis examined 31 randomized trials involving 1,168 participants across stress, attention and cognitive outcomes.
In acute testing, a single 200 mg dose taken 30–60 minutes before testing significantly improved choice reaction time.*
In a separate randomized, placebo‑controlled crossover study, a single 200 mg dose significantly lowered salivary cortisol within 60 minutes compared with placebo.*
PressurePrep includes 200 mg per daily serving.
200 mg
Extensively studied.
View the research (3 studies)
- Cognitive and affective effects of L‑Theanine: a systematic review and meta-analysis of 31 randomized trials →
- Effects of L‑Theanine Administration on Stress-Related Symptoms and Cognitive Functions in Healthy Adults →
- Randomized, placebo-controlled crossover study of a single 200 mg L‑Theanine dose on stress and salivary cortisol →
But L‑theanine alone wasn’t the product we wanted to build.
*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.
L. gasseri CP2305
A different approach to composure—from Japan.
8+ Randomized Human TrialsCP2305 is a heat‑treated postbiotic that produced measurable changes in brain activity, autonomic activity, and self‑reported stress levels in a randomized human trial.*
Human clinical studies of L. gasseri CP2305 have examined its effects on brain activity, parasympathetic activity and mental‑stress measures after a single dose, and on stress response after daily use for up to 24 weeks.*
24‑Week Study
Lower anxiety scores and improved sleep quality*
Medical students under prolonged examination stress
Read study →*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.
CEREBIOME®
Because composure isn’t only about the next hour.
5+ Randomized Human TrialsCEREBIOME® combines Lactobacillus helveticus R0052 and Bifidobacterium longum R0175—the same two proprietary strains used together in randomized human trials that reported improvements in psychological‑distress and stress‑related measures.
In a randomized, placebo‑controlled study, 30 days of these strains significantly improved several psychological‑distress measures and reduced cortisol compared with placebo.*
Why it matters: CEREBIOME adds the longer‑term dimension to PressurePrep, with human research examining consistent use during periods of everyday stress.
30 Days
Significantly improved psychological‑distress measures and reduced cortisol versus placebo.*
View the research (2 studies)
- Assessment of psychotropic-like properties of a probiotic formulation (Lactobacillus helveticus R0052 and Bifidobacterium longum R0175) in rats and human subjects →
- Probiotic food supplement reduces stress-induced gastrointestinal symptoms in volunteers: a double-blind, placebo-controlled, randomized trial →
*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.
Beyond ordinary stress support.
L‑theanine gives PressurePrep a clinically studied, familiar foundation. L. gasseri CP2305 adds an unusual Japanese postbiotic with human research spanning acute and longer‑term stress. CEREBIOME® adds clinically studied probiotics for the consistent‑use side of the formula.
Different ingredients. Different research. One purpose: support greater composure today while building resilience over time.
Today
L‑theanine + L. gasseri CP2305
Calm · Focus · Composure
Consistent Use
L. gasseri CP2305 + CEREBIOME®
Everyday stress resilience
What’s actually inside.
Supplement Facts
Amount Per Serving
% Daily Value
L‑Theanine
200 mg †
Cerebiome® Probiotic Blend
3 billion CFU †
Lactobacillus helveticus R0052
Bifidobacterium longum R0175
L. gasseri CP2305
(Heat-Treated Postbiotic) 20 mg †
(Heat-Treated Postbiotic) 20 mg †
10 billion cells
† Daily Value not established.
Research-aligned amounts of our three core ingredients.
Research is only useful if it helps in real life.
PressurePrep brings together clinically studied ingredients to support calm today and resilience over time.*
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Manufactured in the USA • Made in a GMP‑certified facility • Independently tested